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Updated: Aug 15, 2026
Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
Published on: April 7, 2023
Mesenchymal stem cells from human bone marrow and adipose tissue: isolation, characterization, and differentiation
Yu A Romanov1, A N Darevskaya, N V Merzlikina
1Research and Practical Laboratory of Human Stem Cells, National Complex of Cardiology Research and Practice, Ministry of Health of the Russian Federation, Moscow. romanov@cardio.ru
Abstract:
Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cytofluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III beta-tubulin (neuronal differentiation marker).
