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Updated: Aug 15, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody
Published on: January 24, 2017
[Construction and expression of anti-keratin single-chain Fv antibody]
Guo-min Zhang1, Yu-ping Chen, Gang Wang
1Department of Microbiology and Immunology, Capital University of Medical Science, Beijing 100054, China. erminzhang@sohu.com
Aim:
To construct an expression vector of human anti-keratin scFv and express functional scFv in E. coli.
Methods:
The V(H) and V(L) genes were amplified by PCR and cloned into a scFv expression vector, which was expressed in E. coli. The expression of scFv was tested by SDS-PAGE; The antigen-binding characteristics of scFv were tested by ELISA.
Results:
The V(H) and V(L) genes were cloned and expressed in E. coli successfully. The expressed scFv retained the antigen-binding characteristics of the parental antibody.
Conclusion:
A functional anti-keratin scFv was obtained.
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