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Comparison of a novel thin-layer chromatographic-fluorescence detection method with a spectrofluorometric method for
1Department of Pharmacological Sciences, University of Newcastle upon Tyne, UK.
Journal of Chromatography
|March 27, 1992
Abstract:
A novel method for the determination of 7-hydroxycoumarin in human urine which combines thin-layer chromatography (TLC) with fluorescence detection (FD) has been devised. The limit of detection (1 ng/ml) enables determination of 7-hydroxycoumarin after both administration of coumarin and environmental exposure to this fragrance material. When compared to a spectrofluorometric method of analysis, the TLC-FD method proved to be more selective for the analysis of 7-hydroxycoumarin in human urine.