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Updated: Aug 14, 2026

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells
Published on: March 7, 2025
[TPA increases cell proliferation of HEp-2 cell line in vitro]
Weiwei Liu1, Zongyuan Zeng, Xibin Xiao
1Cancer Center of Sun Yat-Sen University, Guangzhou 510060, China. drliuwei@163.com
Objective:
To investigate the influence of TPA treatment on HEp-2 cell line in vitro.
Method:
HEp-2 cell line cultured in vitro was treated by different concentration levels of TPA. MTT method was used to detect survival cell ratio of HEp-2.
Result:
Cell growth of HEp-2 after treatment of TPA manifested an peak. The highest peak which was 1.3 to 1.4 times of control cell presented on 10 microg/L concentration level. Cell proliferation ability dropped gradually following increase of TPA concentration.
Conclusions:
TPA can increase cell proliferation of HEp-2 cell in vitro.

