Related Experiment Video
Updated: Aug 13, 2026
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
Published on: September 30, 2019
[Selection of the peptides specifically binding to hepatoma by using phage display in vivo]
Bing Du1, Jing Yu, Zhong-liang Zhou
1School of Life Science, East China Normal University, Shanghai 200062, China.
Objective:
To screen the peptides binding to hepatoma specifically.
Methods:
Nude mice were inoculated with human tumor cells BEL-7402, then the Ph.D.-12 Phage Display Peptide Library was injected intravenously (tail vein) into mice. After 20 min the mice were sacrificed and the phage rescued from tumor tissues. All the tissues should be made an appraisal using immunohistochemistry and titering. The phage recovered from the tissues were amplified and purified then re-injected for next round screening. After screening for 3 rounds in vivo, the phage-peptides that homed to the tumor tissues or cells were obtained. Then these phage clones were sequenced to analyze the motif. All the sequenced clones were appraised by using cell ELISA and titering the distribution in vivo.
Results:
Through the appraisal from vivo and vitro and peptides' sequences, several target motifs were preliminarily determined.
Conclusions:
Some phage-peptides which could specifically bind tumor cells or tissues can be selected successfully from the random twelve-peptide library by means of phage display in vivo.
Insights
Researchers successfully identified specific binding peptides for hepatoma cells using in vivo phage display. This method screens a peptide library to find molecules that target cancer tissues effectively.
Area of Science:
- Biotechnology
- Molecular Biology
- Oncology
Context:
- Hepatocellular carcinoma (HCC) is a major global health concern.
- Targeted therapies require specific molecular binders for effective treatment.
- Phage display technology offers a powerful tool for identifying such binders.
Purpose:
- To screen for peptides that specifically bind to hepatoma cells and tissues.
- To identify novel peptide motifs with potential diagnostic or therapeutic applications in HCC.
Summary:
- The study employed an in vivo phage display approach using a twelve-peptide library injected into nude mice bearing BEL-7402 human hepatoma cells.
- Following three rounds of screening, phage clones homing to tumor tissues were isolated, sequenced, and analyzed for target motifs.
- Immunohistochemistry, titering, and cell ELISA were used for appraisal of phage-peptide binding specificity and distribution.
Impact:
- Successfully identified specific phage-peptides that bind to hepatoma cells and tissues.
- Demonstrates the efficacy of in vivo phage display for discovering targeted cancer binders.
- Provides a foundation for developing targeted diagnostic agents or therapeutics for hepatoma.
More Related Videos
08:31Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
Published on: December 1, 2020
07:53Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
Published on: December 10, 2010