Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SU-8 based continuous-flow RT-PCR bio-chips under high-precision temperature control.

Nan-Chyuan Tsai1, Chung-Yang Sue

  • 1Department of Mechanical Engineering, National Cheng Kung University, Taiwan. nortren@mail.ncku.edu.tw

Biosensors & Bioelectronics
|February 9, 2006
PubMed
Summary

A novel micro-reverse transcription polymerase chain reaction (micro-RT-PCR) chip enables quantitative tumor virus detection. This integrated system demonstrates high sensitivity and precise temperature control for improved diagnostic capabilities.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Estimation of Blood Pressure in the Radial Artery Using Strain-Based Pulse Wave and Photoplethysmography Sensors.

Micromachines·2019
Same author

Linear magnetic clutch to automatically control torque output.

ISA transactions·2018
Same author

An integrated thermal compensation system for MEMS inertial sensors.

Sensors (Basel, Switzerland)·2014
Same author

Computer-aided diagnosis for early-stage breast cancer by using Wavelet Transform.

Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society·2010

Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Diagnostics

Background:

  • Advancements in bio-chemical and micro-electro-mechanical systems enable miniaturization of DNA amplification devices.
  • Polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) are key techniques for nucleic acid amplification.
  • Miniaturized devices expedite virus detection processes.

Purpose of the Study:

  • To design and validate a micro-RT-PCR chip for quantitative tumor virus detection.
  • To integrate essential components for nucleic acid analysis onto a single chip.
  • To develop a high-precision temperature control system for the micro-RT-PCR unit.

Main Methods:

  • Fabrication of a SU-8 based monolithic chip integrating test sample reservoirs, RT-PCR meanders, and capillary electrophoresis.

Related Experiment Videos

  • Development of a high-precision temperature control module using signal amplification circuits and an Intel 8051 microprocessor.
  • Utilizing intensive simulations and experimental validation to verify the bio-chip's performance.

    • Main Results:

    • The integrated micro-RT-PCR system demonstrated high efficacy in heat transfer.
    • The chip design effectively prevented fluid flow clogging.
    • Superior sensitivity was achieved in precisely controlling reaction temperatures across all heated zones.
    • Experimental results verified the functionality and validity of the fabricated bio-chip.

    Conclusions:

    • The developed micro-RT-PCR chip offers a promising platform for quantitative tumor virus detection.
    • The integrated system provides precise temperature control and high sensitivity, crucial for accurate diagnostics.
    • This miniaturized approach enhances efficiency and reliability in molecular diagnostics for viral detection.