Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microscale integrated sperm sorter.

Yaokuang Chung1, Xiaoyue Zhu, Wei Gu

  • 1Department of Biomedical Engineering, University of Michigan, Ann Arbor, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 2, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HYPER-Net: Physics-Conditioned Self-Supervised Reconstruction for Fourier Light-Field Microscopy.

bioRxiv : the preprint server for biology·2026
Same author

Interkingdom signaling elicited by bacterial extracellular vesicles in human cystic fibrosis airway epithelium and neutrophils.

Frontiers in cellular and infection microbiology·2026
Same author

Free-floating long-term vascularized mesenchymal organoids.

iScience·2026
Same author

Substrate Exclusion Greenlights Physical Autocatalysis of Enzyme Activity in Membraneless Proto-Organelles.

Biomacromolecules·2025
Same author

Manipulation of the nucleoscaffold potentiates cellular reprogramming kinetics.

PNAS nexus·2025
Same author

A Protocol Guide to Micro Milling for Bio-Microfluidics.

Bio-protocol·2025
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study presents a coin-sized, passively driven microfluidic sperm sorter. The device efficiently separates motile sperm from non-motile sperm and debris for in vitro fertilization and point-of-care diagnostics.

Area of Science:

  • Biomedical Engineering
  • Reproductive Technology
  • Microfluidics

Background:

  • Sperm sorting is crucial for assisted reproductive technologies and diagnostics.
  • Existing methods often require external power sources and complex setups.
  • There is a need for simple, portable, and cost-effective sperm separation devices.

Purpose of the Study:

  • To design and fabricate a passively driven microfluidic sperm sorter.
  • To demonstrate its capability in separating motile from non-motile sperm.
  • To assess its potential for in vitro fertilization and point-of-care applications.

Main Methods:

  • Utilized soft lithographic microfabrication techniques for device fabrication.
  • Incorporated horizontally oriented reservoirs as passive, constant-flow-rate pumps.

Related Experiment Videos

  • Leveraged laminar flow and differential motility for sperm separation.

    • Main Results:

    • Developed a coin-sized, self-contained microfluidic sperm sorting device.
    • Successfully separated motile sperm from non-motile sperm and debris.
    • Demonstrated passive operation without external power sources or controllers.

    Conclusions:

    • The microfluidic sperm sorter is an inexpensive, portable, and effective tool.
    • It simplifies the preparation of high-quality sperm for in vitro fertilization.
    • The device holds promise for convenient sperm motility assays at the point of care or home.