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Updated: Aug 9, 2026

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
Published on: July 17, 2019
Molecular recognition: improved binding of biotin derivatives with synthetic receptors
Fernando Herranz1, M Dolores Santa María, Rosa M Claramunt
1Departamento de Química Organica y Bio-Organica, UNED, Facultad de Ciencias, Senda del Rey 9, E-28040 Madrid, Spain.
Abstract:
NMR titrations and Monte Carlo conformational searches have been used to study the molecular recognition features of five urea derivatives with two synthetic hosts. We have improved the binding constant (Kb) values for all the studied guests and measured the largest binding constant of a complex involving a biotin derivative (biotin methyl ester) bound to a synthetic host by means of several interaction points and not only through the urea moiety.
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