Related Experiment Video

Updated: Aug 9, 2026

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
11:54

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

Published on: July 13, 2019

Transforming activities of JC virus early proteins

Richard J Frisque1, Catherine Hofstetter, Shiva K Tyagarajan

  • 1Pennsylvania State University, University Park, USA.

Advances in Experimental Medicine and Biology
|April 22, 2006
PubMed

Insights

Human polyomaviruses like JC virus (JCV) are small DNA viruses that can cause tumors. This chapter details the five JCV early proteins and their roles in viral oncogenesis, drawing parallels with SV40 and BKV.

Area of Science:

  • Virology
  • Oncology
  • Molecular Biology

Background:

  • Polyomaviruses are small DNA viruses known for their tumor-inducing capabilities in vivo.
  • Human polyomaviruses, such as JC virus (JCV) and BK virus (BKV), along with simian virus 40 (SV40), are key members of this viral family.
  • Research into polyomavirus-host interactions provides critical insights into cellular processes and viral oncogenesis.

Purpose of the Study:

  • To elucidate the roles of the five known JC virus (JCV) early proteins in the oncogenic process.
  • To detail how each JCV early protein contributes to cellular transformation when expressed in cultured cells.
  • To supplement understanding of JCV protein function with data from SV40 and BKV studies where JCV-specific information is lacking.

Main Methods:

  • Focus on the functional analysis of JCV early proteins.
  • Utilize cell culture systems to study protein expression and cellular transformation.
  • Incorporate comparative analysis with related polyomaviruses like SV40 and BKV.

Main Results:

  • The chapter details the specific contributions of each of the five JCV early proteins to viral oncogenesis.
  • Demonstrates how these proteins induce cellular transformation in experimental settings.
  • Highlights conserved and distinct functions among JCV, SV40, and BKV early proteins.

Conclusions:

  • The five JCV early proteins play distinct roles in the viral oncogenic process.
  • Understanding these proteins is crucial for comprehending polyomavirus-induced tumorigenesis.
  • Comparative studies with SV40 and BKV enhance our knowledge of polyomavirus molecular mechanisms.

More Related Videos

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy06:40

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

Related Concept Videos

Inhibitors of Virion Maturation and Assembly01:19

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...
Retroviruses02:33

Retroviruses

Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...