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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
One-flask synthesis of dinucleoside tetra- and pentaphosphates
Qianwei Han1, Barbara L Gaffney, Roger A Jones
1Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, 08854, USA.
Organic Letters
|May 5, 2006
Abstract:
[reaction: see text] We report a one-flask route for the synthesis of dinucleoside tetra- and pentaphosphates, in isolated yields of 50-85%. This route relies on a mixture of P(III) and P(V) chemistries, using phosphitylation of a protected nucleoside with 2-chloro-4H-l,3,2-benzo-dioxaphosphorin-4-one (salicylchlorophosphite), followed by sequential reaction with inorganic pyrophosphate and a nucleoside 5' mono- or diphosphate.
