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Norepinephrine stimulates calprotectin expression in human monocytic cells
Suryono1, Jun-ichi Kido, Noriko Hayashi
1Department of Periodontology, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia.
Journal of Periodontal Research
|May 9, 2006
Summary
Stress hormone norepinephrine significantly increases calprotectin expression in human monocytic cells, suggesting stress modulates calprotectin levels alongside inflammation.
Area of Science:
- Immunology
- Stress Physiology
- Oral Biology
Background:
- Calprotectin (S100A8/S100A9) is elevated in inflamed gingival tissues.
- Periodontal disease is linked to psychological stress.
- Stress mediators like norepinephrine and cortisol influence disease pathways.
Purpose of the Study:
- To investigate the impact of stress mediators, norepinephrine and cortisol, on calprotectin expression.
- To explore the cellular mechanisms underlying stress-induced calprotectin modulation.
Main Methods:
- U-937 human monocytic cells were treated with norepinephrine or cortisol.
- Gene and protein expression of calprotectin (S100A8/S100A9) were analyzed via northern blotting and ELISA.
- Transcription factor C/EBPalpha DNA binding activity was assessed using electrophoretic mobility shift assay.
Main Results:
- Norepinephrine significantly upregulated S100A8/S100A9 mRNA and increased calprotectin protein by 3.6-fold via beta-adrenergic receptors.
- Cortisol did not affect calprotectin expression at the mRNA or protein level.
- Norepinephrine enhanced C/EBPalpha DNA binding activity, while cortisol did not.
Conclusions:
- Norepinephrine, a key stress modulator, stimulates calprotectin expression in human monocytic cells.
- Stress, mediated by norepinephrine, represents an additional regulatory factor for calprotectin expression beyond inflammation.