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Related Experiment Videos

Personal cytometers: slow flow or no flow?

Howard M Shapiro1, Nancy G Perlmutter

  • 1The Center for Microbial Cytometry, West Newton, MA 02465-2513, USA. hms@shapirolab.com

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|May 9, 2006
PubMed
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A low-cost personal cytometer under $5,000 is feasible using readily available components. This imaging cytometer can detect low levels of fluorescence for broad cytomics applications.

Area of Science:

  • Biotechnology
  • Medical Devices
  • Cytometry

Background:

  • Current
  • personal cytometers
  • cost over $40,000, hindering accessibility.
  • A true personal cytometer, analogous to personal computers, should cost under $5,000.
  • Such affordable instruments are needed for cytomics in diverse global settings.

Purpose of the Study:

  • To assess the technical and economic feasibility of developing a low-cost personal cytometer.
  • To explore the potential of an imaging cytometer for broad cytomics applications.

Main Methods:

  • Evaluated high-intensity LEDs as fluorescence excitation sources.
  • Utilized inexpensive CCD cameras as detectors.
  • Employed 35 mm camera lenses and plastic microscope optics for light collection in a low-resolution imaging cytometer.

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  • Tested components using resolution targets and fluorescent bead standards.

    • Main Results:

    • Developed an instrument capable of detecting fewer than 10,000 molecules of cell-associated fluorescent label.
    • Demonstrated applicability to a wide range of cytometric tasks.

    Conclusions:

    • An affordable personal cytometer under $5,000 is achievable.
    • An imaging cytometer is more likely to meet the cost target than a flow cytometer.
    • Component availability supports the development of cost-effective cytometers.