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Updated: Aug 7, 2026

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry
Published on: December 16, 2022
Golli protein negatively regulates store depletion-induced calcium influx in T cells
Ji-Ming Feng1, Yanhong K Hu1, Lai-Hua Xie2
1Semel Institute of Neuroscience and Human Behavior, UCLA Geffen School of Medicine, 635 Charles Young Drive, Los Angeles, California 90095.
Abstract:
Calcium influx is crucial for T cell activation and differentiation. The detailed regulation of this process remains unclear. We report here that golli protein, an alternatively spliced product of the myelin basic protein gene, plays a critical role in regulating calcium influx in T cells. Golli-deficient T cells were hyperproliferative and showed enhanced calcium entry upon T cell receptor stimulation. We further found that golli regulates calcium influx in T cells through the inhibition of the store depletion-induced calcium influx. Mutation of the myristoylation site on golli disrupted its association with the plasma membrane and reversed its inhibitory action on Ca2+ influx, indicating that membrane association of golli was essential for its inhibitory action. These results indicate that golli functions in a unique way to regulate T cell activation through a mechanism involving the modulation of the calcium homeostasis.
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