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Updated: Aug 4, 2026

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
Published on: June 23, 2012
A biased detection of the flexible genome content: missing large restriction fragments in comparative genomics
1Department of Biochemistry and Biophysics, University of Arizona, Tucson, AZ 85721, USA. mvpassel@email.arizona.edu <mvpassel@email.arizona.edu>
Abstract:
Gene content variation between closely related bacteria is largely due to mobile elements and gene erosion. Various techniques are available to assess this variation, yet biased pools of restriction fragments have been used in several comparative genomics studies. Excluding important large restriction fragments from the actual hybridisation or from post-hybridisation amplification reactions compromise the comprehensiveness of these studies.
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