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[A method for determining urea by using immobilized urease].

I P Ivanov, S I Danev, Ch D Palashev

    Laboratornoe Delo
    |January 1, 1990
    PubMed
    Summary
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    A new method using immobilized urease offers faster, more sensitive, and reagent-saving urea measurements in biological fluids. This technique is reliable and ideal for rapid analysis, especially in small labs.

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Clinical Diagnostics

    Background:

    • Urea measurement is crucial for diagnosing various medical conditions.
    • Existing methods like the phenolhypochlorite method can be slow and reagent-intensive.
    • There is a need for more efficient and sensitive urea detection techniques.

    Purpose of the Study:

    • To develop and validate a novel manual method for urea determination in biological fluids.
    • To compare the performance of the new method against established urea assays.
    • To assess the suitability of the method for routine laboratory use.

    Main Methods:

    • Urease enzyme was immobilized onto sepharose gel beads.
    • Biological fluid samples were analyzed for urea content using the immobilized urease method.

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  • Results were compared with those obtained from the phenolhypochlorite method (soluble urease) and the diacetylmonoxime method.

    • Main Results:

    • The immobilized urease method demonstrated significantly higher sensitivity (twice) compared to the soluble urease method.
    • The new technique was 3-6 times faster and more reagent-saving, with reagents reusable up to 500 times.
    • High correlation coefficients (r = 0.9988 and 0.995) were observed between the immobilized urease method and the other two methods, indicating high analytical reliability.

    Conclusions:

    • Immobilized urease in sepharose gel provides a superior method for manual urea measurement.
    • The method is efficient, sensitive, cost-effective, and reliable for clinical diagnostics.
    • This technique is particularly advantageous for rapid urea analysis in resource-limited or small laboratory settings.