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Updated: Aug 12, 2026
07:12
Neutrophil Isolation Protocol
Published on: July 23, 2008
[A method of separating mononuclears on a density gradient]
Laboratornoe Delo
|January 1, 1990
Abstract:
The developed procedure for the isolation of mononuclears in density gradient d = 1.0077 g/cm3 consists in separation of the plasma by preliminary centrifugation at 200 g for 7-10 min and isolation of leukocytic mononuclear cells from the cellular sediment diluted with the isotonic solution. The length of centrifugation at 400 g is selected with consideration for the thickness of the density gradient. An anticoagulant is added to the isotonic solution and ficoll-verografin mixture to improve the quality of the cell isolation.
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