Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Setup a model for study the interfere between chemokine and immunocyte].

Mike Min1, Chaowu Ma, Yuqin Zhan

  • 1Department of Otolaryngology, No. 117 Hospital of PLA, Hangzhou 310004, China. meek@263.net

Lin Chuang Er Bi Yan Hou Ke Za Zhi = Journal of Clinical Otorhinolaryngology
|September 1, 2006
PubMed
Summary

Recombinant human TNF-alpha (rhTNF-alpha) attracts tonsil B lymphoblastic cell lines (TB-LCL) in a dose-dependent manner. This chemoattraction model aids in studying chemokine and immunocyte interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Molecular Switch Regulating Cell Fate Choice between Muscle Progenitor Cells and Brown Adipocytes.

Developmental cell·2017
Same author

Microarray analysis of differentially expressed genes and their functions in omental visceral adipose tissues of pregnant women with vs. without gestational diabetes mellitus.

Biomedical reports·2017
Same author

Development and validation of a simplified titration method for monitoring volatile fatty acids in anaerobic digestion.

Waste management (New York, N.Y.)·2017
Same author

Association Analysis of Nonsyndromic Congenital Heart Disease and Tag Single Nucleotide Polymorphisms of TBX20 and Genes in the Ras-MAPK Pathway.

Genetic testing and molecular biomarkers·2017
Same author

Poly (fumaroyl bioxirane) maleate: A potential functional scaffold for bone regeneration.

Materials science & engineering. C, Materials for biological applications·2017
Same author

[Cone-beam CT evaluation of volume and surface area changes of impacted upper central incisors before and after orthodontic treatment].

Shanghai kou qiang yi xue = Shanghai journal of stomatology·2017

Area of Science:

  • Immunology
  • Cell Biology

Context:

  • Cytokines play crucial roles in immune cell regulation.
  • Tumor necrosis factor-alpha (TNF-alpha) is a key inflammatory cytokine.
  • B cell lymphoblastic cell lines provide a model for studying B cell behavior.

Purpose:

  • To investigate the chemoattractant effect of recombinant human TNF-alpha (rhTNF-alpha) on tonsil B lymphoblastic cell lines (TB-LCL).
  • To establish a model system for studying cytokine-B cell interactions.

Summary:

  • Tonsil B lymphoblastic cell lines (TB-LCL) were generated by transfecting PTC with Epstein Barr virus.
  • rhTNF-alpha demonstrated a dose-dependent chemotactic effect on TB-LCL.
  • The chemotactic effect was inhibited by the neutralizing antibody E6 against TNF.

Related Experiment Videos

Impact:

  • This study presents a valuable model for investigating chemokine and immunocyte interactions.
  • Understanding cytokine-mediated B cell migration is crucial for immunology research.