Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

4Pi microscopy deconvolution with a variable point-spread function.

David Baddeley1, Christian Carl, Christoph Cremer

  • 1Kirchhoff Institut für Physik, Universität Heidelberg, Germany. david.baddeley@kip.uni-heidelberg.de

Applied Optics
|September 2, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lamin B1 safeguards the B cell genome and shapes lymphoma outcome.

HemaSphere·2026
Same author

Single-molecule localization microscopy imaging of extracellular vesicle DNA in recipient cells.

Journal of translational medicine·2026
Same author

A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context.

bioRxiv : the preprint server for biology·2025
Same author

Cell aging - a relevant factor in live cell microscopy (mini-review).

Progress in biophysics and molecular biology·2025
Same author

Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications.

Journal of cell science·2025
Same author

Perspective Article: Space-time dynamics of genome replication studied with super-resolved microscopy.

Postepy biochemii·2024
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

This study introduces a new deconvolution method for 4Pi microscopy that corrects for phase shifts in the point spread function (PSF). This technique improves image quality by accurately handling varying optical conditions within samples.

Area of Science:

  • Microscopy
  • Optical Imaging
  • Image Processing

Background:

  • 4Pi microscopy requires deconvolution to remove axial sidelobes.
  • High axial resolution makes 4Pi point spread functions (PSFs) sensitive to optical imperfections.
  • Spatially invariant PSF deconvolution fails with significant phase shifts.

Purpose of the Study:

  • To develop a deconvolution technique for 4Pi microscopy that accounts for spatially varying phase shifts.
  • To enable accurate image reconstruction despite imperfect optical conditions.

Main Methods:

  • A technique for computing the forward transformation with varying phase was developed.
  • A method for estimating the PSF phase from acquired images was implemented.
  • A deconvolution procedure was constructed based on these novel techniques.

Related Experiment Videos

Main Results:

  • The new method computes the forward transformation with computational cost similar to shift-invariant methods.
  • The technique effectively estimates and corrects for phase variations in the PSF.
  • Deconvolution procedures were successfully built upon the developed phase estimation and computation methods.

Conclusions:

  • The presented techniques enable robust deconvolution in 4Pi microscopy even with spatially varying phase shifts.
  • This advancement improves the reliability and accuracy of 4Pi imaging under challenging optical conditions.