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Updated: Jul 19, 2026
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Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
Published on: May 16, 2019
[Study on vitrification of porcine embryos by open pulled straw method]
De-Fu Zhang1, Dong Liu, Hua-Li Wu
1Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, China. zhangdefu10@yahoo.com.cn
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|October 14, 2006
Summary
This study demonstrates successful piglet birth from vitrified embryos using a two-step method. This research marks a significant advancement in pig embryo cryopreservation and reproductive technologies in China.
Area of Science:
- Veterinary Science
- Reproductive Biology
- Cryobiology
Context:
- Vitrification is a key cryopreservation technique for embryos.
- Efficient methods for pig embryo vitrification are crucial for swine reproduction.
- Previous studies have explored various vitrification protocols for porcine embryos.
Purpose:
- To evaluate the efficacy of a two-step vitrification method using open pulled straws (OPS) for porcine embryos.
- To assess the viability and developmental potential of vitrified-then-warmed embryos.
- To establish a successful live birth protocol for vitrified pig embryos in China.
Summary:
- 291 porcine embryos (blastocyst/morula stage) were vitrified using a two-step method with OPS in ethylene glycol (EG) and dimethyl sulfoxide (DMSO) solutions.
- Embryos were stored in liquid nitrogen for 3 months, then warmed and transferred to 8 recipient sows.
- One recipient sow became pregnant, resulting in the birth of 8 live piglets.
Impact:
- This study reports the first successful live birth of piglets from vitrified embryos in China.
- The findings validate the effectiveness of the described two-step vitrification protocol for porcine embryos.
- This research contributes to advancing assisted reproductive technologies in swine breeding and conservation efforts.
