[An immunoenzyme method of detecting natural antibodies to thrombin and alpha 2-macroglobulin]

An enzyme immunoassay technique was developed for the detection of natural IgG and IgM antibodies to thrombin and alpha 2-macroglobulin in normal donor plasma and serum. The optimal conditions for the detection of IgG antibodies to alpha 2-macroglobulin are the same for the blood plasma and serum. IgG antithrombin antibodies are detectable in the plasma within a small range of the sorbed antigen concentrations, and if the serum is analyzed, this range is lowered by two orders. Natural antibody levels to the studied antigens were found to be constant in a person.