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Entrapment of proteins by aggregation within sephadex beads
S K Khare1, S Vaidya, M N Gupta
1Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas.
Applied Biochemistry and Biotechnology
|March 1, 1991
Abstract:
When a protein is aggregated by chemical crosslinking inside Sephadex beads of appropriate pore size, it gets trapped inside the beads. The above approach was used for immobilization of beta-galactosidase, acid phosphatase, trypsin, and concanavalin A. It was found to be a simple, convenient, and fast method for immobilization of proteins.