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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
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From Gateway to MultiSite Gateway in one recombination event

Enrico Magnani1, Linnea Bartling, Sarah Hake

  • 1Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA. emagnani@berkeley.edu

BMC Molecular Biology
|December 8, 2006
PubMed
Summary

This study introduces a new tool to expand the applications of MultiSite Gateway cloning. The pDONR-R4-R3 construct converts existing Gateway vectors, making this versatile cloning technology more accessible for diverse biological research.

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