Related Experiment Video
Updated: Jul 18, 2026

Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
The molecular-thermometer based on B-Z transition of DNA
Ryu Tashiro1, Hiroshi Sugiyama
1Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.
Abstract:
A molecular thermometer utilizing the different pi-stacking of B- and Z-DNA was designed. Aminopurine, which is a fluorescent probe for charge-transfer processes, was introduced into B- and Z-DNA and the emission monitored. Z-DNA showed a marked increase in fluorescence compared with B-DNA, with the fluorescence intensity correlating linearly and reproducibly with temperature.
Related Concept Videos
DNA as a Genetic Template
Modern Molecular Taxonomy
The DNA Helix
The DNA Helix
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
PCR

