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Updated: Jul 18, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Direct subcloning of target region from BAC insert using restriction enzymes that produce non-identical cohesive ends

Yuhsuke Ohmi1, Masahiro Sato, Masato Ohtsuka

  • 1Division of Basic Molecular Science and Molecular Medicine, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.

Nucleic Acids Symposium Series (2004)
|December 8, 2006
PubMed

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