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Updated: Jul 18, 2026
Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing
Yoshio Saito1, Keiichiro Kanatani, Yuji Ochi
1NEWCAT Institute, Faculty of Engineering, Nihon University and SORST, Japan Science and Technology Corporation, Tamura, Koriyama 963-8642, Japan.
Abstract:
We have devised novel pyrene-labeled BDF nucleosides, (Py)U, (Py)C, (Py)deazaA, and (Py)A. BDF probes containing these fluorescent nucleosides selectively emit fluorescence only when the base opposite the BDF base is a target base. Oligonucleotides containing these BDF nucleosides act as effective reporter probes for homogeneous SNP typing of DNA samples.
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