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Updated: Jul 18, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Kinetics and thermodynamics of G-quadruplexes
Julien Gros1, Mateus Webba da Silva, Anne De Cian
1Laboratoire de Biophysique, INSERM U565, CNRS UMR5153, Muséum National d'Histoire Naturelle, 43 rue Cuvier, 75005 Paris, France.
Abstract:
The melting of tetramolecular DNA or RNA quadruplexes is kinetically irreversible. However, rather than being a hindrance, this kinetic inertia allows us to study association and dissociation processes independently. General rules have been extended to longer DNA motifs or sequences containing modified bases such as 8-oxo or 7-deaza guanine. Results were compared with the canonical TG4T and TG5T tetramers: we demonstrate huge differences (up to 10(5)-fold) in the association constants of these quadruplexes depending on primary sequence.
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