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Updated: Jul 18, 2026

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
Published on: November 4, 2025
Design of light-switchable phage promoter for efficient photo-regulation of gene-expression
Mingzhe Liu1, Makoto Komiyama, Hiroyuki Asanuma
1Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Abstract:
Efficient photoregulation of transcription by T7 RNA polymerase (RNAP) has been achieved with modified T7-promoter involving two azobenzene moieties at RNAP recognition region and TATA region simultaneously. Transcription proceeded 7.5-fold faster after UV irradiation than that under dark condition. Kinetic parameters (kcat, Km) for the transcription revealed that an azobenzene at each position played a different role and thus synergistic effect of photo-regulation was achieved with the promoter involving two azobenzenes.
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