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Updated: Jul 18, 2026
Mouse Eye Enucleation for Remote High-throughput Phenotyping
Published on: November 19, 2011
[Experimental studies on ocular tissues taken from mice of electrocution under the electron microscope]
Hualan Jing1, Yuming Lu, Yong Fu
1Department of Forensic Medicine, Preclinical school of Sun Yat-sen University, 510080, China. hljin@gzsums.edu.cn
Purpose:
To observe morphological characteristics in ocular tissues with electrocution under the electron microscope.
Methods:
Fourteen mice are divided into two groups, the experimental group and the control group. In the experimental group, all of the 9 mice are killed by electricity (220 v, 50 Hz), with the heads connected to anode and the right leg to cathode. In the control group, all of the 5 mice are beheaded. Eyes fixed in glutaraldehyde, are split into two halves. One is observed under the electron microscope, and the other is observed morphological characteristics by H-E stain, which has been published.
Results:
In the experimental group, morphological characteristics of the components of eyes present in varied degrees under the electron microscope, but not obvious in control group.
Conclusion:
It can be drawn that these certain morphological characteristics in the experimental group might be helpful to the treatment of electrical injury.
Insights
Electrocution causes distinct changes in ocular tissues, observable via electron microscopy. These findings may aid in treating electrical injuries to the eye.
Area of Science:
- Ophthalmology
- Toxicology
- Electron Microscopy
Context:
- Electrocution is a significant cause of accidental injury.
- Understanding the specific tissue damage is crucial for effective treatment.
- Ocular tissues are particularly vulnerable to electrical trauma.
Purpose:
- To investigate the ultrastructural changes in ocular tissues following electrocution using electron microscopy.
- To identify specific morphological alterations indicative of electrical injury in the eye.
Summary:
- Mice exposed to electrocution (220 V, 50 Hz) exhibited varied morphological changes in ocular tissues under electron microscopy.
- Control mice, subjected to decapitation, showed no significant alterations.
- These observed ultrastructural differences highlight the impact of electrical current on eye components.
Impact:
- The identified morphological characteristics provide insights into the pathophysiology of electrocution-induced ocular damage.
- Findings may contribute to developing targeted therapeutic strategies for electrical eye injuries.
- This research offers a basis for further investigation into the long-term effects and treatment of electrical trauma to the eye.
