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Updated: Jul 18, 2026

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Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
Published on: September 28, 2016
[Expression and characterization of VPAC2 in CHO cells]
Rong-Jie Yu1, Yuan Gao, Yun Dai
1Bio-engineering Institute of Jinan University, Guangzhou 510632, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|December 16, 2006
Summary
Researchers developed a Chinese Hamster Ovary (CHO) cell line stably expressing the VPAC2 receptor. This new cell line, VPAC2-CHO, demonstrates high sensitivity to PACAP38 and is crucial for studying VPAC2 receptor functions.
Area of Science:
- Molecular Biology
- Cell Biology
- Pharmacology
Context:
- The VPAC2 receptor plays a vital role in mediating the biological functions of pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP).
- Understanding VPAC2 receptor activity is essential for various physiological processes.
- Stable expression systems are required for detailed receptor characterization.
Purpose:
- To construct a stable Chinese Hamster Ovary (CHO) cell line expressing the VPAC2 receptor.
- To validate the functional expression and sensitivity of VPAC2 in the engineered cell line.
- To establish a platform for future research on VPAC2 and the development of related therapeutics.
Summary:
- The study successfully transfected CHO cells with pcDNA-VPAC2 and selected stable clones using G418.
- The clone VPAC2-CHO exhibited high sensitivity to PACAP38, evidenced by increased cAMP production.
- Multiple assays, including RT-PCR, Western blot, immunofluorescence, and Scatchard analysis, confirmed effective VPAC2 expression and functionality, with a receptor density of (1.1 +/- 0.2) pmol/mg and Kd of (0.55 +/- 0.10) nmol/L for PACAP38.
Impact:
- Provides a robust CHO cell line (VPAC2-CHO) for specialized research on VPAC2 receptor.
- Facilitates in-depth investigation into the characteristics and functions of VPAC2.
- Enables the screening and characterization of novel agonists and antagonists targeting the VPAC2 receptor.
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