Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Typing mycobacteria using the specific inhibitor method].

E A Bibergal'

    Laboratornoe Delo
    |January 1, 1991
    PubMed
    Summary

    A new radionuclide method quickly identifies Mycobacterium tuberculosis strains, including M. bovis and M. humanis group, using thiophene-2-carboxylic acid as an inhibitor. This technique aids in rapid mycobacterial strain differentiation for improved diagnostics.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [The effect of human plasma and cattle blood serum on the culture properties of liquid nutrient media].

    Laboratornoe delo·1991
    Same author

    [Protein spectrum of mycobacteria in relation to their taxonomy and resistance to antitubercular drugs].

    Problemy tuberkuleza·1991
    Same author

    [Determination of resistance of Mycobacterium tuberculosis to pyrazinamide by radiometric method].

    Problemy tuberkuleza·1991
    Same author

    [Comparative study of the effect of preservatives on the viability of the Mycobacterium populations].

    Problemy tuberkuleza·1991
    Same author

    [Transamination processes in mycobacteria with different biological properties].

    Problemy tuberkuleza·1990
    Same author

    [Use of 3H-thymidine to study the metabolism of Mycobacterium tuberculosis as changed during chemotherapy].

    Problemy tuberkuleza·1988

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Context:

    • Accurate identification of Mycobacterium tuberculosis complex (MTBC) strains is crucial for effective treatment and epidemiological control.
    • Current methods for strain differentiation can be time-consuming, delaying appropriate patient management.
    • Distinguishing between M. bovis and other members of the M. tuberculosis complex, such as the M. tuberculosis humanis group, is diagnostically important.

    Purpose:

    • To develop and validate a rapid radionuclide-based method for differentiating MTBC strains.
    • To assess the efficacy of thiophene-2-carboxylic acid as a selective inhibitor for strain identification.
    • To enable timely assignment of isolates to M. bovis, M. bovis R-INH, or MOTT (Mycobacteria other than tuberculosis) groups.

    Summary:

    • A radionuclide-based assay was developed to differentiate MTBC strains within four days.
    • The method relies on measuring the ratio of population growth indices in the presence and absence of thiophene-2-carboxylic acid.
    • This approach allows for the classification of strains into M. bovis, M. bovis R-INH, or MOTT categories.

    Impact:

    • Provides a faster alternative to existing mycobacterial strain identification techniques.
    • Facilitates quicker clinical decisions and public health interventions.
    • Enhances the ability to track and manage tuberculosis and related mycobacterial infections.

    Related Experiment Videos