Related Experiment Video
Updated: Jul 17, 2026
Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
Published on: September 22, 2017
[Artifacts in optical coherence tomography (OCT) imaging of the retina]
R W Strauss1, F Scholz, M W Ulbig
1Augenklinik der Ludwig-Maximilians-Universität, München.
Purpose:
The purpose of this study was to investigate artifacts of OCT scans and of software analysis for retinal cross-section scans in a specialised retina clinic setting.
Methods:
A total of 205 vertical cross-hair OCT scans of retinal Stratus OCTs were randomly chosen from the database. All scans had been performed by one experienced technician. There were 75 (37%) normal findings, the remaining scans showed various types of retinal pathology: All scanning artifacts were analysed. Retinal thickness of all scans was measured automatically at the centre of the macula using two different software algorithms: the instrument's built-in "Stratus OCT Viewer V 4.01" and the stand-alone application "Datamedical OCTview V 3.5" (Datamedical Consulting, Hamburg). Errors of the software to correctly identify the retinal surface and the outer highly reflective layer were assigned into three categories: none, minor error (no influence on measurements) and major error.
Results:
A total of 7.3% of all OCT scans showed scanning artifacts: 5 motion artifacts, 9 scans with low signal intensity and 1 decentred scan. Scanning artifacts significantly increased the frequency of software errors (p = 0.012). The presence of retinal pathology also increased the number of errors (p = 0.004). Software analysis yielded a total of 20 major and 2 minor errors for the Stratus OCT (overall 10.7%) and 32 major and 64 minor errors for the Datamedical Viewer (p < 0.001). Measurements by Datamedical OCTview were a mean of 57 micron higher due to a different definition of the outer retinal border. Retinal pathologies significantly increased the likelihood of software errors for both algorithms (both p < 0.01), most critical were macular holes and changes in age-related macular degeneration.
Conclusion:
Scanning artifacts were associated with a significantly higher frequency of software errors. As artifacts of scans and software occur frequently, the interpretation of OCT scans requires special attention to artifacts.
