Related Experiment Video
Updated: Jul 16, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
Published on: March 7, 2018
Developing global insight into RNA regulation
1Howard Hughes Medical Institute, Laboratory of Molecular Neuro-Oncology, The Rockefeller University, New York, New York 10021, USA.
Abstract:
Systematic dissection of the activity of RNA-binding proteins (RBPs) has begun to yield global insight into how they work. The paradigm we have used has been the study of Nova, a neuron-specific RBP targeted in an autoimmune neurologic disorder associated with cancer. We have developed a combination of biochemical, genetic, and bioinformatic methods to generate a global understanding of Nova's role as a splicing regulator. Genome-wide identification and validation of Nova target RNAs have yielded unexpected insights into the protein's mechanism of action, its role in neurobiology, and the unique roles RBPs have in the biology of the neuronal synapse. These studies provide us with a paradigm for understanding the role of RBPs in neurons and in disease and, more generally, with the hope that it will be feasible to develop a comprehensive understanding of posttranscriptional regulation.
Related Concept Videos
Regulation of Expression at Multiple Steps
Translational Regulation
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Types of RNA
RNA Performs Diverse...

