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Augmentation of acute random pattern skin flap viability in the pig
A Zhong1, C Y Pang, W D Sheffield
1Division of Surgical Research, Hospital for Sick Children, Toronto, Ontario, Canada.
The Journal of Surgical Research
|February 1, 1992
Summary
Ketanserin and LY53857, S2-serotonergic receptor antagonists, significantly improved skin flap blood flow and viability in pigs. This study provides the first evidence that S2-serotonergic receptors play a role in skin flap ischemia.
Area of Science:
- Regenerative Medicine
- Vascular Surgery
- Pharmacology
Background:
- Acute random pattern skin flaps are susceptible to ischemia.
- S2-serotonergic receptors are implicated in vascular regulation.
- Limited research exists on the role of S2-serotonergic receptors in skin flap survival.
Purpose of the Study:
- To investigate the effect of ketanserin and LY53857, S2-serotonergic receptor antagonists, on skin flap blood flow and viability.
- To determine the therapeutic potential of these antagonists in improving skin flap survival.
Main Methods:
- Three experiments were conducted on porcine skin flaps (4 x 10 cm).
- Intravenous ketanserin dose-response on capillary blood flow using radioactive microspheres.
- Intramuscular ketanserin and LY53857 treatment for five days on skin flap viability.
Main Results:
- Intravenous ketanserin (0.25-0.35 mg/kg) significantly increased skin flap blood flow.
- Both ketanserin and LY53857 significantly increased the length of skin flap viability compared to controls.
- Early post-operative ketanserin treatment also enhanced skin flap viability.
Conclusions:
- Ketanserin and LY53857 significantly augment porcine acute random pattern skin flap viability.
- This study provides the first experimental evidence for S2-serotonergic receptor involvement in skin flap ischemia pathogenesis.
- S2-serotonergic receptor antagonists represent a potential therapeutic strategy for improving skin flap survival.