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Updated: Jul 15, 2026
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Quantitative proteomic analysis of mammalian organisms using metabolically labeled tissues
Christine C Wu1, Michael J MacCoss
1Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 9, 2007
Summary
Stable isotope metabolic labeling generates tissue-specific standards for quantitative proteomics. This method complements existing techniques for analyzing protein expression in mammalian organisms.
Area of Science:
- Biochemistry
- Proteomics
- Metabolomics
Background:
- Quantitative proteomic analyses require accurate internal standards.
- Stable isotope labeling offers a novel approach for generating these standards.
- This method provides an alternative to chemical labeling techniques.
Purpose of the Study:
- To detail the methodology for metabolic labeling of mammalian organisms with stable isotopes.
- To describe the application of Multidimensional Protein Identification Technology for analyzing labeled samples.
- To outline the computational analysis of mass spectrometry data.
Main Methods:
- Metabolic labeling of mammalian organisms using stable isotopes.
- Sample preparation and analysis via Multidimensional Protein Identification Technology (MuPIT).
- Computational processing of acquired mass spectrometry data.
Main Results:
- Successful generation of isotope-labeled tissues.
- Acquisition of mass spectrometry data using MuPIT.
- Establishment of a computational pipeline for data analysis.
Conclusions:
- Metabolic labeling is a viable strategy for producing internal standards in quantitative proteomics.
- This technique is complementary to other labeling methods.
- The described workflow enables robust proteomic analysis of labeled organisms.
