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Related Experiment Videos

Rebuilding microbial genomes.

Robert A Holt1, Rene Warren, Stephane Flibotte

  • 1Canada's Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada. rholt@bcgsc.ca

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|May 18, 2007
PubMed
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Creating synthetic cells by rebuilding genomes is feasible. Researchers overcame obstacles in DNA transfer, replication, and gene regulation when assembling a synthetic Haemophilus influenzae genome in an Escherichia coli host.

Area of Science:

  • Synthetic biology
  • Microbial engineering
  • Genomics

Background:

  • Engineered microbes offer significant potential in biotechnology and research.
  • Synthetic cells can be created by assembling small molecules or by synthesizing and assembling a genome.

Purpose of the Study:

  • To examine obstacles in creating a synthetic cell by reassembling a fragmented genome in a host cell.
  • To assess the feasibility of rebuilding and booting a synthetic genome.

Main Methods:

  • Utilized a model system with a donor genome from Haemophilus influenzae.
  • Fragmented, modified, and reassembled the genome stepwise within an Escherichia coli host cell.

Main Results:

  • Identified obstacles related to DNA transfer, replication, gene regulation cross-talk, and gene product compatibility.

Related Experiment Videos

  • Observed widespread transcription of Haemophilus influenzae genes in Escherichia coli.
  • Found few genes incompatible with the E. coli host.

    • Conclusions:

    • Rebuilding and booting a genome is a pragmatic approach for creating synthetic microbial cells.
    • Despite challenges, the strategy demonstrates feasibility for synthetic cell construction.