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Updated: Jul 14, 2026
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A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood
Published on: August 15, 2025
A comparative study of 2 methods for obtaining platelet-rich plasma.
Faleh M Tamimi1, Santiago Montalvo, Isabel Tresguerres
1Department of Stomatology III, Faculty of Odontology, Complutense University of Madrid, Madrid, Spain.
Summary
The ACE double-centrifugation method yields higher platelet concentrations for platelet-rich plasma (PRP) than the Nahita single-centrifugation system. However, ACE shows more variability and potential ultrastructural alterations in PRP.
Area of Science:
- Biomaterials Science
- Dental Regenerative Medicine
- Hematology
Background:
- Platelet-rich plasma (PRP) is widely used in dentistry.
- Double and single centrifugation are common methods for PRP preparation.
- Comparing PRP preparation techniques is crucial for optimizing clinical outcomes.
Purpose of the Study:
- To compare the ACE double-centrifugation system with the Nahita single-centrifugation system for obtaining platelet-rich plasma (PRP).
- To evaluate platelet yield and ultrastructural integrity of PRP prepared by two different centrifugation methods.
Main Methods:
- Blood samples from 30 patients were processed using both the ACE and Nahita systems.
- Platelet counts were determined by flow cytometry.
- Ultrastructural analysis of PRP was performed using transmission electron microscopy (TEM).
Main Results:
- The ACE system achieved a higher platelet concentration (336%) compared to the Nahita system (227%).
- ACE system results showed greater dispersion in platelet counts than the Nahita system.
- TEM revealed significant alterations in platelet aggregates with the ACE system, particularly if not mixed in the final stage, while Nahita PRP showed normal physiological appearance.
Conclusions:
- The ACE double-centrifugation method provides higher platelet yields but with increased variability.
- The ACE system can cause ultrastructural changes in PRP and is more susceptible to preparation errors.
- The Nahita single-centrifugation system offers a more consistent PRP preparation with preserved platelet morphology.
