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Long-term observation of pial microcirculatory parameters using an implanted cranial window method in the rat

Hiroshi Masuda1, Akira Ushiyama, Shogo Hirota

  • 1Department of Environmental Health, National Institute of Public Health, 2-3-6 Minami, Wako-shi, Saitama, 351-0197 Japan. msd@niph.go.jp

In Vivo (Athens, Greece)
|June 27, 2007
PubMed
Summary

Our improved closed cranial window (CCW) method enables long-term intravital microscopy of rat pial microcirculation. This technique maintains normal blood-brain barrier permeability, leukocyte behavior, and plasma velocities for up to four weeks post-implantation.

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