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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
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Cellular image analysis and imaging by flow cytometry.

David A Basiji1, William E Ortyn, Luchuan Liang

  • 1Amnis Corporation, Seattle, 2505 Third Avenue, Suite 210, WA 98121, USA. dbasiji@amnis.com

Clinics in Laboratory Medicine
|July 31, 2007
PubMed
Summary

Imaging flow cytometry merges flow cytometry

Area of Science:

  • Biomedical Imaging
  • Cell Biology
  • Analytical Chemistry

Background:

  • Standard flow cytometry lacks spatial resolution.
  • Digital microscopy offers spatial detail but lacks high throughput.
  • Integrating these techniques addresses limitations in cell analysis.

Purpose of the Study:

  • To present imaging flow cytometry as a tool for clinical cell analysis.
  • To demonstrate its application in distinguishing cancerous from normal cells.
  • To showcase high-throughput analysis of fluorescence in situ hybridization (FISH) probes.

Main Methods:

  • Utilizing imaging flow cytometry to capture cell images and quantitative data.
  • Applying the technique to analyze mammary epithelial cells for cancer detection.

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Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
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  • Performing high-throughput quantitation of FISH probes in human peripheral blood mononuclear cells.

    • Main Results:

    • Successful discrimination between cancerous and normal mammary epithelial cells.
    • Efficient high-throughput quantitation of FISH probes in blood cells.
    • Demonstrated potential for clinical applications in bodily fluid analysis.

    Conclusions:

    • Imaging flow cytometry is suitable for clinical applications, analyzing cells in bodily fluids.
    • The technique enables discrimination of cell types and quantitation of molecular probes.
    • Future integration with extended depth-of-field imaging will further enhance FISH analysis.