Related Experiment Video
Updated: Jul 13, 2026

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
Published on: July 22, 2008
Xist function: bridging chromatin and stem cells
1Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, 1030 Vienna, Austria. wutz@imp.univie.ac.at
Abstract:
In mammals, dosage compensation is achieved by transcriptional silencing of one of the two female X chromosomes. X inactivation is dynamically regulated in development. The non-coding Xist RNA localizes to the inactive X, initiates gene repression in the early embryo, and later stabilizes the inactive state. Different functions of Xist are observed depending on which epigenetic regulatory pathways are active in a given cell. Because Xist has evolved recently, with the origin of placental mammals, the underlying pathways are also important in regulating developmental control genes. This review emphasizes the opportunity that Xist provides to functionally define epigenetic transitions in development, to understand cell identity, pluripotency and stem cell differentiation.
More Related Videos
12:42Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
Published on: November 26, 2014
10:23Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
Published on: February 26, 2015
Related Concept Videos
Inheritance of Chromatin Structures
Chromatin Modification in iPS Cells
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
X-Inactivation
X-inactivation
Maintenance of the ES Cell State
Methods of Nuclear Reprogramming