Related Experiment Video
Updated: Jul 13, 2026

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
Published on: June 26, 2018
[Pyrogallol red technique is an alternative to routine urinary protein-determining methods]
Abstract:
The paper presents the basic urinary protein-determining methods, such as turbidimetric and colorimetric ones and provides their brief analytical characterization. The authors consider the sulfosalicylic technique as most universally employed by clinical diagnostic laboratories and the pyrogallolic one as most promising. The comparative characteristics of both techniques are described and their advantages and disadvantages are noted. In terms of correct estimation and reproducibility of the results of an analysis, the pyrogallic technique is superior to the sulfosalicylic and other methods for protein determination; it can identify microproteinuria and thus a better alternative to the routine methods for urinary protein determination.
Related Concept Videos
Differential Staining Technique
Urine Studies I: Urinalysis
Imaging Studies V: Intravenous Urography and Retrograde Pyelography
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.