Related Experiment Video
Updated: Jul 13, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
Published on: January 26, 2011
Clathrin-associated adaptor proteins - putting it all together
Adaptor proteins link clathrin coats to membrane budding sites. This study speculates on how adaptor protein subunits assemble into functional complexes based on sequence features.
Area of Science:
- Cell biology
- Structural biology
- Protein biochemistry
Background:
- Adaptor proteins function as crucial linkers in coated vesicle formation.
- They connect the clathrin lattice to the membrane during vesicle budding.
- The precise assembly of adaptor protein subunits into functional complexes remains unclear.
Purpose of the Study:
- To explore the assembly of adaptor protein subunits.
- To propose a speculative low-resolution structural model for subunit interactions.
Main Methods:
- Analysis of protein sequence features.
- Development of a speculative structural model.
Main Results:
- Identified sequence features relevant to subunit interactions.
- Proposed a low-resolution model for adaptor protein complex assembly.
Conclusions:
- Sequence analysis provides insights into adaptor protein quaternary structure.
- A speculative model aids in understanding functional complex formation.
More Related Videos
12:40Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
Published on: October 20, 2014
10:02Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
Published on: October 23, 2016
Related Concept Videos
Clathrin Coated Vesicles
Coat Assembly and GTPases
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Pinching-off of Coated Vesicles
Adherens Junctions
Adherens Junctions are Dynamic
The endothelial cells...
Intracellular Signaling Affects Focal Adhesions
Some...
Catenins
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...