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Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

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Published on: February 1, 2018

Recent developments in potentiometric biosensors for biomedical analysis.

Robert Koncki¹

¹University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland. rkoncki@chem.uw.edu.pl

Analytica Chimica Acta

|September 4, 2007

Summary

Potentiometric biosensors show promise for biomedical analysis, particularly for detecting urea and creatinine. This review highlights recent advancements in bioaffinity-based sensors for immunoassays and genoanalysis, discussing their practical applications and limitations.

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Area of Science:

Electrochemistry
Biosensor Technology
Biomedical Analysis

Background:

Potentiometric biosensors utilize biocatalytic and bioaffinity schemes.
Few biosensors are currently suitable for direct biomedical analysis.
Urea and creatinine detection represent key applications in protein metabolism monitoring.

Purpose of the Study:

To review recent trends in potentiometric biosensor development.
To discuss the advantages, limitations, and challenges of these biosensors.
To highlight potentiometric biosensors applicable to real biomedical analysis.

Main Methods:

Review of existing literature on potentiometric biosensors.
Analysis of biocatalytic and bioaffinity-based sensing strategies.
Evaluation of biosensors for urea, creatinine, immunoassays, and genoanalysis.

Main Results:

Bioaffinity-based potentiometric biosensors are emerging for immunoassays and genoanalysis.
Biosensors for urea and creatinine detection are particularly promising for clinical use.
Recent trends focus on improving sensitivity, selectivity, and real-world applicability.

Conclusions:

Potentiometric biosensors offer significant potential for biomedical diagnostics.
Further research is needed to overcome limitations for widespread clinical adoption.
Specific applications in urea and creatinine monitoring demonstrate current viability.