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Related Experiment Video

Updated: Jul 11, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

A modular tissue engineering construct containing smooth muscle cells and endothelial cells.

Brendan M Leung1, Michael V Sefton

  • 1Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, M5S 3G9, Toronto, ON, Canada.

Annals of Biomedical Engineering
|September 21, 2007
PubMed
Summary

Serum-free culture of umbilical vein smooth muscle cells (UVSMC) improves endothelial cell junction morphology and function in vascular constructs. This finding is crucial for modulating blood vessel construct thrombogenicity.

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Area of Science:

  • Biomaterials Science
  • Vascular Tissue Engineering
  • Cell Biology

Background:

  • Human umbilical vein endothelial cells (HUVEC) and umbilical vein smooth muscle cells (UVSMC) are key components in vascular tissue engineering.
  • Developing vascularized constructs requires understanding cell-cell interactions and material influences.
  • Modulating the thrombogenicity of engineered blood vessels is a critical challenge.

Purpose of the Study:

  • To investigate the impact of embedded UVSMC, cultured under different serum conditions, on HUVEC behavior within collagen modules.
  • To assess how UVSMC phenotype, influenced by serum concentration, affects HUVEC junction morphology, proliferation, and nitric oxide (NO) secretion.
  • To explore the potential of UVSMC in modulating the thrombogenicity of HUVEC-based vascular constructs.

Main Methods:

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Fabrication of Myogenic Engineered Tissue Constructs
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Fabrication of Myogenic Engineered Tissue Constructs

Published on: May 1, 2009

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Last Updated: Jul 11, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Fabrication of Myogenic Engineered Tissue Constructs
13:43

Fabrication of Myogenic Engineered Tissue Constructs

Published on: May 1, 2009

  • Human umbilical vein endothelial cells (HUVEC) were seeded onto sub-millimeter collagen cylinders containing embedded umbilical vein smooth muscle cells (UVSMC).
  • UVSMC were cultured in media with varying serum concentrations (10% FBS vs. serum-free) prior to embedding.
  • HUVEC junction morphology, proliferation, NO secretion, and module contraction were assessed based on the UVSMC culture conditions.

Main Results:

  • Embedding UVSMC cultured in 10% FBS medium adversely affected HUVEC junction morphology.
  • UVSMC cultured in serum-free medium prior to embedding improved HUVEC junction morphology and promoted HUVEC proliferation and NO secretion.
  • Serum-free UVSMC culturing prevented UVSMC-induced contraction of the collagen modules.

Conclusions:

  • The phenotype of embedded UVSMC significantly influences the behavior of seeded HUVEC in vascular constructs.
  • Serum concentration in the UVSMC culture medium is a critical factor for modulating HUVEC phenotype and construct stability.
  • These findings provide a basis for utilizing UVSMC to control the thrombogenicity of engineered vascular tissues.