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Related Experiment Video

Updated: Jul 11, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Published on: November 26, 2008

Separation-based glycoprofiling approaches using fluorescent labels.

Paula Jane Domann¹, Ana Carmen Pardos-Pardos, Daryl Ludger Fernandes

¹LGC Ltd, Teddington, Middlesex, UK. paula.domann@lgc.co.uk

|September 26, 2007

Summary

This summary is machine-generated.

Glycoprotein analysis is crucial for biopharmaceutical safety and efficacy. This study compares normal phase chromatography and capillary gel electrophoresis for analyzing complex released glycans, aiding method development.

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Last Updated: Jul 11, 2026

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A Method of Targeted Cell Isolation via Glass Surface Functionalization

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Published on: September 20, 2016

Area of Science:

Biopharmaceutical analysis
Analytical chemistry
Glycobiology

Background:

Glycoprotein analysis is vital for biopharmaceutical product safety and efficacy.
Analyzing released glycans is challenging due to complexity, lack of chromophores, and isoforms.
Glycoproteins are heterogeneous, leading to diverse glycan structures in analysis.

Purpose of the Study:

To compare normal phase (NP) chromatography and capillary gel electrophoresis with laser induced fluorescence (CGE-LIF) for analyzing fluorescently labeled glycans.
To evaluate these methods against industry-established criteria for biopharmaceutical applications.
To provide insights for developing and validating glycan analysis methods.

Main Methods:

Normal phase (NP) chromatography: sensitive, reliable, established with retention time databases.
Capillary gel electrophoresis with laser induced fluorescence (CGE-LIF): offers faster analysis but lacks mobility-based databases.
Cross-correlation of CGE-LIF data with NP chromatography or mass spectrometry is necessary for method validation.

Main Results:

Both NP chromatography and CGE-LIF demonstrate excellent selectivity for fluorescently labeled glycans.
NP chromatography benefits from existing databases for structure identification.
CGE-LIF requires complementary techniques for robust structural assignment.

Conclusions:

Both NP chromatography and CGE-LIF are valuable techniques for biopharmaceutical glycan analysis.
The choice of method depends on specific analytical needs, balancing speed, established databases, and validation requirements.
Further development of databases for CGE-LIF could enhance its standalone utility.