Related Experiment Video

Updated: Jul 10, 2026

Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth
08:36

Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth

Published on: May 31, 2024

Disparity between Yersinia pestis and Yersinia enterocolitica O:8 in YopJ/YopP-dependent functions

Ayelet Zauberman1, Baruch Velan, Emanuelle Mamroud

  • 1Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona.

Advances in Experimental Medicine and Biology
|October 31, 2007
PubMed

Insights

Yersinia effector proteins YopJ and YopP disrupt immune responses. A comparative study revealed differences in their effects on immune cells, with YopJ

Area of Science:

  • Microbiology
  • Immunology
  • Pathogen Biology

Background:

  • Yersinia effector proteins YopP (in Y. enterocolitica) and YopJ (in Y. pseudotuberculosis) modulate host immune responses.
  • These proteins are known to suppress cytokine expression and induce apoptosis in host cells, contributing to pathogen virulence.

Purpose of the Study:

  • To comparatively analyze the in vitro effects of YopJ/YopP from Yersinia species on immune cells.
  • To investigate the role of YopJ in the virulence of Yersinia pestis during bubonic plague.

Main Methods:

  • In vitro comparative analysis of Yersinia pestis and Yersinia enterocolitica O:8 virulent strains.
  • Evaluation of YopJ involvement in a mouse model of bubonic plague.

Main Results:

  • Significant disparities were observed in the effects of YopJ/YopP on immune cells between the studied Yersinia strains.
  • The study provides insights into the differential modulation of host immune responses by these key Yersinia virulence factors.

Conclusions:

  • YopJ and YopP exhibit distinct mechanisms of action on immune cells, despite their conserved functions.
  • The findings highlight the complex interplay between Yersinia effectors and host immunity, crucial for understanding plague pathogenesis.

More Related Videos

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions12:23

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Published on: October 13, 2015

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

Related Concept Videos

Plague01:24

Plague

Plague is a highly virulent zoonotic disease caused by Yersinia pestis, a Gram-negative, facultatively anaerobic coccobacillus. This pathogen primarily circulates among rodent populations and is transmitted to humans through the bite of infected fleas. Additional transmission routes include direct contact with infected animal tissue or inhalation of respiratory droplets from individuals with pneumonic plague. These multiple transmission pathways highlight the bacterium’s potential for rapid...
Determinants of Bacterial Pathogenicity and Virulence01:20

Determinants of Bacterial Pathogenicity and Virulence

Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
Regulation of Bacterial Virulence01:28

Regulation of Bacterial Virulence

Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...