Related Experiment Video
Updated: Jul 10, 2026

08:46
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Published on: January 6, 2015
Cloning and expression of rat GFAP cDNA in Escherichia coli
V P Chekhonin1, K A Pavlov, A N Shkoporov
1Laboratory of Immunochemistry, Department of Biological Psychiatry, V. P. Serbskii Center of Social and Forensic Psychiatry, Moscow.
Bulletin of Experimental Biology and Medicine
|November 21, 2007
Abstract:
A cDNA fragment encoding GFAP was amplified by reverse transcription PCR from total mRNA isolated from primary culture of rat astrocytes and cloned for expression in Escherichia coli using pET-28a vector. High level of GFAP expression was confirmed by SDS-PAGE, while immunochemical identity was verified by immunoblotting. The constructed producer strain is a cheap source of GFAP and can be used for diagnostic purposes.

