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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Construction of target molecule sensing system using aptameric enzyme subunit based on PQQGDH activity
Kazunori Ikebukuro1, Yo Morita, Wataru Yoshida
1Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan. ikebu@cc.tuat.ac.jp
Nucleic Acids Symposium Series (2004)
|November 22, 2007
Summary
Researchers developed a novel aptameric enzyme subunit (AES) for sensing. This new AES uses pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) to detect adenosine, demonstrating a new biosensing capability.
Area of Science:
- Biochemistry
- Molecular Biology
- Biosensor Technology
Background:
- A novel target sensing element, the aptameric enzyme subunit (AES), was previously developed.
- AES combines target-binding aptamers with aptamers that modulate enzymatic activity.
- Previous work demonstrated adenosine and DNA sensing using thrombin-based AES.
Purpose of the Study:
- To screen for pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) aptamers that modulate PQQGDH activity.
- To construct a novel AES utilizing a PQQGDH aptamer for target detection.
- To establish a model system for adenosine detection using the PQQGDH-based AES.
Main Methods:
- Screening of PQQGDH aptamers for activity modulation.
- Construction of an AES by linking a PQQGDH aptamer with an adenosine aptamer.
- Detection of adenosine by measuring PQQGDH activity changes upon target binding.
Main Results:
- Successfully screened PQQGDH aptamers that influence PQQGDH enzymatic activity.
- Constructed a functional AES using a PQQGDH aptamer and an adenosine aptamer.
- Demonstrated adenosine detection through measurable changes in PQQGDH activity.
Conclusions:
- The PQQGDH-based AES is a viable tool for target molecule detection.
- This approach expands the application of AES in biosensing.
- The developed AES system offers a novel method for adenosine quantification.
