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Updated: Jul 9, 2026
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
Published on: October 15, 2013
Separation and simultaneous determination of uric acid and ascorbic acid on a dynamically modified
Jianding Qiu1, Pengfei Hu, Ruping Liang
1Department of Chemistry, Nanchang University, Nanchang 330031, P. R. China. jdqiu@ncu.edu.cn
This study presents a novel microfluidic chip for separating and detecting uric acid and ascorbic acid simultaneously. The developed method offers sensitive and reproducible analysis of these compounds in human urine samples.
Area of Science:
- Analytical Chemistry
- Microfluidics
- Electrochemistry
Background:
- Simultaneous separation and detection of uric acid and ascorbic acid are crucial for clinical diagnostics.
- Microfluidic devices offer advantages in sample handling and analysis speed.
Purpose of the Study:
- To develop and optimize a poly(dimethylsiloxane) microfluidic chip for the simultaneous separation and electrochemical detection of uric acid and ascorbic acid.
- To evaluate the performance of the developed method for analyzing these analytes in human urine.
Main Methods:
- Fabrication of poly(dimethylsiloxane) microfluidic channels with alternating polyelectrolyte multilayers (poly(diallyldimethylammonium chloride)/poly(sodium 4-styrenesulfonate)).
- Coupling of the modified microchip with in-channel electrochemical detection.
- Optimization of separation conditions, including buffer solution, detection potential, and separation voltage.
Main Results:
- Successful simultaneous separation and detection of uric acid and ascorbic acid.
- Optimal linear ranges of 25–600 µM for both analytes with high correlation coefficients (0.997 for uric acid, 0.996 for ascorbic acid).
- Low detection limits: 8 µM for uric acid and 5 µM for ascorbic acid.
- Demonstrated stability, sensitivity, and reproducibility of the microfluidic device.
Conclusions:
- The developed polyelectrolyte-modified microfluidic chip coupled with electrochemical detection provides an effective platform for the simultaneous analysis of uric acid and ascorbic acid.
- The method is suitable for the determination of uric acid and ascorbic acid in complex biological matrices like human urine.
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