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Related Experiment Video

Updated: Jul 9, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system.

Linfen Yu1, Zheng Shen, Jiankun Mo

  • 1Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Electrophoresis
|December 12, 2007
PubMed
Summary

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This study introduces a rapid on-chip electrophoresis system for measuring individual cell electrophoretic mobility (EPM). The system effectively differentiates drug-treated cells, showing potential for clinical applications.

Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Cell Biology

Background:

  • Electrophoretic mobility (EPM) is a key cell surface property.
  • Traditional methods for EPM measurement are time-consuming.
  • A need exists for high-throughput cell analysis systems.

Purpose of the Study:

  • To develop and validate a simple on-chip electrophoresis system for individual cell EPM measurement.
  • To simultaneously characterize cell electrophoresis behavior and fluorescence signals.
  • To assess the system's utility in distinguishing drug-treated cells.

Main Methods:

  • Utilized an on-chip electrophoresis system with LIF multipoint detection.
  • Analyzed dye-labeled K562 cells and K562 cells treated with arsenic trioxide (As2O3).

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Last Updated: Jul 9, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

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Published on: December 10, 2011

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

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  • Measured migration time and distance from electropherograms of individual cells.

    • Main Results:

    • Demonstrated distinct EPM differences between untreated and As2O3-treated K562 cells.
    • Observed lower EPM in K562 cells after drug treatment, concentration-dependent.
    • Achieved high throughput, analyzing over 300 cells within 2 hours.

    Conclusions:

    • The developed system provides a simple, fast, and high-throughput method for EPM analysis.
    • EPM measurement can effectively differentiate cellular responses to anticancer drugs.
    • The system shows promise for evaluating cell surface properties in clinical and pharmaceutical settings.