Related Experiment Video
Updated: Jul 9, 2026

11:08
Fabrication And Characterization Of Photonic Crystal Slow Light Waveguides And Cavities
Published on: November 30, 2012
Collinear type II second-harmonic-generation frequency-resolved optical gating for use with high-numerical-aperture
D N Fittinghoff1, J A Squier, C P Barty
1Institute for Nonlinear Science, University of California, San Diego, La Jolla, California 92093-0339, USA.
Optics Letters
|December 19, 2007
Summary
Characterizing ultrashort laser pulses at a microscope
Area of Science:
- Optics and Photonics
- Laser Physics
- Microscopy
Background:
- Ultrashort-pulse lasers are crucial for multiphoton microscopy.
- Optimizing laser performance necessitates pulse characterization at the microscope objective's focal point.
Purpose of the Study:
- To develop and demonstrate a method for characterizing ultrashort laser pulses at the tight focus of a microscope objective.
- To enable full utilization of the microscope objective's numerical aperture (N.A.) during pulse characterization.
Main Methods:
- Utilized a collinear frequency-resolved optical gating (FROG) geometry.
- Employed type II second-harmonic generation (SHG) for pulse characterization.
- Integrated the FROG technique with a standard microscope objective.
Main Results:
- Successfully measured the intensity and phase of a 22-femtosecond (fs) laser pulse.
- Demonstrated the technique using a 20x, 0.4-N.A. air objective.
- Validated the collinear FROG method for in-situ pulse analysis.
Conclusions:
- The developed collinear FROG technique effectively characterizes ultrashort pulses at the microscope focal plane.
- This method allows for the full numerical aperture of microscope objectives to be leveraged.
- Accurate pulse characterization is essential for optimizing multiphoton microscopy systems.
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

